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. 2019 May 10;101(2):347–359. doi: 10.1093/biolre/ioz089

Figure 6.

Figure 6.

Spo11P306T/P306T spermatocytes have fewer MLH1 foci, indicative of reduced crossovers. (A) Chromosome spreads of pachytene spermatocytes immunolabeled with MLH1 and SYCP3. Size bar = 20μM. (B) Quantification of MLH1 foci. Actual numbers are as follows: WT (n = 3, cells = 118, avg = 23.7 ± 0.12), and Spo11P306T/P306T (n = 4, cells = 108, avg = 19.6 ± 0.2). Avg ± SEM. (C)Spo11P306T/P306T spermatocytes have an increased incidence of chromosomes lacking an apparent crossover (MLH1 focus). N = 3 mice for each genotype. For WT and mutant, 102 and 58 cells were scored, respectively. All statistics were done using paired Student t-test. Avg ± SEM.