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. 2019 May 10;101(2):347–359. doi: 10.1093/biolre/ioz089

Figure 7.

Figure 7.

Pre-DSB recombinosomes persist on chromosome axes of Spo11P306T/P306T and Spo11P306T/−. (A) Chromosome surface spreads of spermatocytes immunolabeled with MEI4 and SYCP3. Size bar = 20μm. (B) Quantification of MEI4 foci at different phases of prophase I. Actual numbers are as follows. Leptonema: WT (n = 2 mice, cells = 27, avg = 163 ± 7.1), Spo11P306T/P306T (n = 3, cells = 25, avg = 169 ± 4.1), and Spo11P306T/- (n = 2, cells = 29, avg = 188 ± 7.9); early zygonema: WT (n = 2, cells = 32, avg = 29.7 ± 5.1), Spo11P306T/P306T (n = 3, cells = 25, avg = 44 ± 4.3) and Spo11P306T/- (n = 2, cells = 35, avg = 86 ± 5.9); late zygonema: WT (n = 2, cells = 51, avg = 1.2 ± 0.26), Spo11P306T/P306T (n = 3, cells = 48, avg = 2.8 ± 5.9) and Spo11P306T/- (n = 2, cells = 38, avg = 45 ± 4.5). Pachynema: WT (n = 2, cells = 51, avg = 1.2 ± 0.3), Spo11P306T/P306T (n = 3, cells = 48, avg = 2.8 ± 5.9), and Spo11P306T/- (n = 2, cells = 38, avg = 45.4 ± 4.5). (C) Chromosome spreads of pachytene spermatocytes immunolabeled with IHO1 and SYCP3. Size bar = 20μm. (D) Percentage of early and late pachytene and diplotene spermatocytes with indicated IHO1 localization patterns. Early pachynema: WT (n = 2, cells = 40) and Spo11P306T/P306T (n = 3, cells = 60); late pachynema: WT (n = 2, cells = 30) and Spo11P306T/P306T (n = 3, cells = 60); diplonema: WT (n = 2, cells = 30) and Spo11P306T/P306T (n = 3, cells = 30). Aut = autosomes; PT = Spo11P306T/P306T. All statistics were done using paired Student t-test. Avg ± SEM.