Figure 4.

miR-221-knockdown suppresses IAV-triggered type-I IFN production in A549 cells. (A and B) Cell and supernatant were harvested at 0, 12 and 24 h post-infection, and then ELISA assay was performed to measure IFN-α and IFN-β expression. (C-F) Reverse transcription quantitative-PCR was performed to measure the expression of IFN-stimulated genes (MxA, OAS, viperin and PKR). Data are presented as the mean ± SD of three individual experiments. **P<0.01 vs. inhibitor NC group. NC, negative control; IAV, influenza A virus; miR, microRNA; OAS, 2′-5′-oligoadenylate synthase 2; MxA, myxovirus influenza resistance 1; PKR, interferon-inducible double-stranded RNA-dependent protein kinase activator A.