Figure 2.

Effects of PYCP on myotube diameter in TNF-α-treated C2C12 myotubes. Representative images and quantification of myotube diameters are shown for C2C12 myotubes treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Images were captured at ×20 magnification (scale bar, 50 µm). Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 vs. untreated cells; ^#P<0.05 vs. TNF-α-only treated cells. PYCP, Pyropia yezoensis protein; TNF-α, tumor necrosis factor-α.