Figure 3.

Effects of PYCP on the production of intracellular ROS in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Intracellular ROS production was measured using DCF-DA and fluorescence intensity analysis. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 vs. untreated cells; ^#P<0.05 vs. TNF-α-only treated cells. PYCP, Pyropia yezoensis protein; ROS, reactive oxygen species; TNF-α, tumor necrosis factor-α.