Figure 5.

Effects of PYCP on the activation and translocation of NF-κB/p65 in TNF-α-treated C2C12 myotubes. (A) p-IκBα, IκBα, and NF-κB/p65 protein expression levels of cytosolic fractions were detected by western blot analysis. (B) NF-κB/p65 levels were measured in the nuclear fractions by western blot analysis. β-actin and lamin B1 were used as internal controls for the cytosolic and nuclear fractions, respectively. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 vs. untreated cells; ^#P<0.05 vs. TNF-α-only treated cells. PYCP, Pyropia yezoensis protein; NF-κB, nuclear factor-κB; TNF-α, tumor necrosis factor-α; p-, phosphorylated; IκBα, inhibitor of κB α.