Figure 6.

Effects of PYCP on the ubiquitin-proteasome system in TNF-α-treated C2C12 myotubes. (A) The atrogin-1/MAFbx and MuRF1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. (B) 20S proteasome activity was assessed by detecting AMC in cell lysates after cleavage from the AMC-tagged peptide LLVY. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 vs. untreated cells; ^#P<0.05 vs. TNF-α-only treated cells. PYCP, Pyropia yezoensis protein; TNF-α, tumor necrosis factor-α; MAFbx, muscle atrophy F-box; MuRF1, muscle RING-finger protein-1; AMC, 7-amino-4-methylcoumarin.