Table I.
List of primary antibodies used in western blot analysis.
| Antibody | Catalog number | Species of origin | Dilution |
|---|---|---|---|
| Atrogin-1/MAFbx | sc-27645 | Goat | 1:1,000 |
| β-actin | sc-47778 | Mouse | 1:1,000 |
| GAPDH | sc-25778 | Mouse | 1:1,000 |
| IκBα | sc-7218 | Rabbit | 1:1,000 |
| Lamin B1 | sc-377000 | Mouse | 1:1,000 |
| MuRF1 | sc-27642 | Goat | 1:1,000 |
| MyoD | sc-377186 | Mouse | 1:1,000 |
| Myogenin | sc-12732 | Mouse | 1:1,000 |
| NF-κB p65 | sc-8008 | Mouse | 1:500 |
| p-IκBα | sc-8404 | Mouse | 1:1,000 |
| TNF-R1 | sc-8436 | Mouse | 1:1,000 |
All antibodies were purchased from Santa Cruz Biotechnology, Inc. MAFbx, muscle atrophy F-box; IκBα, inhibitor of κB α; MuRF1, muscle RING-finger protein-1; MyoD, myoblast determination protein 1; NF-κB, nuclear factor-κB; p-, phosphorylated; TNF-R1, TNF receptor-1.