Figure 1.

Characterization of HFD-induced NAFLD model. (A) Representative images of livers from mice fed HFD or CD. (B) Liver paraffin sections from C57BL/6 mice (n=5) fed HFD or CD for 12 weeks were used to assess liver damage (HE), fibrosis by detecting collagen deposition (Masson and Sirius red staining), cell death (TUNEL staining) and pathological angiogenesis (CD31 staining). (C) Serum ALT levels were measured in mice fed HFD or CD (n=5). ***P<0.001. HFD, high-fat diet; CD, chow diet; HE, hematoxylin-eosin; NAFLD, non-alcoholic fatty acid liver disease; NAS, non-alcoholic steatohepatitis activity score; ALT, alanine aminotransferase.