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. 2021 May 6;24(1):498. doi: 10.3892/mmr.2021.12137

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Characterization of hepatocyte-released EVs. (A) Representative transmission electron microscopy images of morphology of EVs isolated from mice fed HFD or CD. (B) Size distribution of EVs was analyzed using nanoparticle tracking analysis. (C) Markers of EVs (CD9, calreticulin, HSP70, TSG101 and GAPDH) were measured in cells and EVs using western blot analysis. EVs, extracellular vesicles; HFD, high-fat diet; CD, chow diet; HSP70, heat shock protein 70; TSG101, tumor susceptibility 101.