Table 2.

Nonhistone Targets of EZH2, Summarized

Substrate	Site	Peptide	Function and notes	Reference
β-Catenin	K49me3	LSGGNP	Promotes repression of Sox1 and Sox3 in ESCs, competes with acetylation at the same site	81
EloA	K754me	TVKIAP	Modulates expression of low-expression PRC2 target genes in mouse ESCs	82
Fra-2	K104me, K104me2	GVITIG	May prevent Fra-2 from transcriptionally regulating epidermal differentiation genes	83
GATA4	K299me	LYMLHG	Reduces its transcriptional activity by preventing p300 acetylation in HL-1 cardiac muscle cells	84
Histone H2B	K120me	AVTYTS	May compete with H2BK120-ub in vitro and in cancer cell lines	85
Jarid2	K116me3, K116me2	AQRFAQ	Mimics trimethylated histone tail and allosterically activates PRC2 in ESCs	86,87
p38α	K139me3, K165me3	RGLYIH CELILD	May regulate p38 activity	48 and unpublished data
PLZF	K430me	SGMTYG	Based on predictive software∗	88
RORα	K38me	SARSEP	Promotes ubiquitination and degradation in HEK293 cells	89
Stat3	K49me2	AASESH	Promotes transcription of STAT3 target genes in response to IL-6 in colon cancer cells	90
Stat3	K180me3	KTLSQG	Promotes activation of STAT3 signaling in glioblastoma stem-like cells	12
Talin	K2454me3	EAMRLQ	Disrupts binding of Talin to F-actin in neutrophils and dendritic cells	91

Shown are the putative nonhistone targets of EZH2, along with the sequences of the three amino acids.

ESC, embryonic stem cell.

^∗Prediction of Methylation Sites Based on Enhanced Feature Encoding Scheme (PMeS).