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. 2021 May 17;18:37. doi: 10.1186/s12970-021-00435-3

Table 1.

Overview of the subjects, strategies, performance changes and PEDro scores of the included studies

Study Subjects VO2max (ml·kg− 1·min− 1) ”Train-low” strategy Changes in endurance performance PEDro score
Yeo et al. 2008 [40]

14 male cyclists

Low: n = 7

High: n = 7

Low: 60

High: 61

”Twice-a-day” every second day vs. “Once-a-day” every day

6 sessions (3 AT and 3 HIIT) per week for 3 weeks

HIIT commenced with low vs. high muscle glycogen

Athletes instructed to consume of 8-9 g CHO ·kg− 1·day− 1 throughout the period

Total training volume: 7 h·week− 1

Low = High

60 min preload + 60 min TT

10–12% increase in PO during TT in both groups (P < 0.01)

5
Hulston et al. 2010 [41]

14 male cyclists

Low: n = 7

High: n = 7

Low: 65

High: 66

”Twice-a-day” every second day vs. “Once-a-day” every day

6 sessions (3 AT and 3 HIIT) per week for 3 weeks

HIIT commenced with low vs. high CHO availability

Athletes instructed to consume high CHO diet throughout the period

Total training volume: 7.5 h·week− 1

Low = High

60 min preload + 1017 ± 73 kJ TT

10–11% increase in PO during TT in both groups (P < 0.001)

6

Cox et al.

2010 [42]

16 male triathletes

Low: n = 8

High: n = 8

Groups combined:

65

“Fasted training” vs. training with high CHO availability

6 sessions per week for 23 days

5 g CHO·kg− 1·day− 1 in both groups

High: +  1.5 g CHO·kg− 1·day− 1 for every hour of exercise

Low: +  25 kJ·kg− 1·day− 1 for every hour of exercise from fat and protein

Low: fasting for 2 h prior to and during all sessions

High: ingestion of CHO before or during all sessions

Low = High

100 min preload + 7 kJ/kg TT

4–6% reduced time in TT in both groups (P < 0.01)

6
Marquet et al. 2016 [43]

21 male triathletes

Low: n = 11

High: n = 10

Low: 60

High: 60

”Sleep-low”

3x CHO periodization per week for 3 weeks

Identical diets in both groups but different timing

0 g vs. 5 g CHO·kg− 1 during and between afternoon and morning sessions

Additional AT sessions in both groups

Total training volume: 10-15 h·week− 1

Low > High

40 km bike preload + 10 km run

− 2.9% run time in Low (P < 0.01)

− 0.1% run time in High (ns)

6
Marquet et al. 2016 [44]

21 male cyclists

Low: n = 12

High: n = 9

Groups combined:

64

”Sleep-low”

3x per week for 1 week

Identical diets in Low and High around “train-low” sessions but different timing

0 vs. 5 g CHO·kg− 1 between afternoon HIIT and morning AT.

Total training volume: 5 h during the 6-day training period.

Low > High

120 min preload + 20 km TT

− 3% TT time in Low (P < 0.05)

− 1% TT time in High (ns)

6
Burke et al. 2017 [6]

19 male elite race walkers

Low: n = 10

High: n = 9

Low: 65

High: 62

“Fasted training”, “Sleep-low” and “Twice-a-day”

Alternating strategies 6 days per week for 3 weeks

Identical energy and CHO intake low and high but different timing.

Average total training volume: Low: 125 km·week− 1; High: 117 km·week− 1

Low = High

10 km race walking

5–7% reduced walking time in both groups (P < 0.01)

5
Gejl et al. 2017 [45]

26 male triathletes and cyclists

Low: n = 13

High: n = 13

Low: 65

High: 65

”Twice-a-day”

6 sessions (3 HIT and 3 AT) per week for 4 weeks

1 g vs. 6 g CHO · kg− 1 between HIT and LIT sessions (isocaloric diets)

Additional AT sessions to attain habitual training volume

Average total training volume in matched pairs of athletes: 16 h·week− 1

Low = High

90 min preload + 30 min TT

5–6% increase in PO during TT in both groups (P = 0.0003)

6
Riis et al. 2019 [46]

13 male endurance athletes

Low: n = 6

High: n = 7

Low: 63

High: 65

”Sleep-low”

6 sessions (3 HIT and 3 AT) per week for 4 weeks

Identical diets but different timing.

0 vs. 3.6 g CHO·kg− 1 between afternoon HIIT and morning AT

Total training volume: 6hrs45min·week− 1

Low = High

90 min preload + 30 min TT

14–19% increase in PO during TT in both groups (P = 0.005)

7
Burke et al. 2020 [8]

16 elite race walkers

Low:

n = 5 males

n = 3 females

High:

n = 5 males

n = 3 females

Low: 59

High: 58

“Fasted training”, “Sleep-low” and “Twice-a-day”

Alternating strategies 6 days per week for 25 days

Identical energy intake in Low and High but different timing

Average total training volume: 113 and 106 km·week− 1 in Low and High.

Low = High

10 km race walk

− 2.2% walk time in Low (P = 0.09)

− 4.8% walk time in High (P < 0.001)

5

AT moderate-to-high intensity aerobic training, CHO carbohydrate, High control group receiving CHO, HIIT high intensity interval training, LIT low intensity training session, Low “train-low” group exposed to periodized fasting or CHO restriction, MAP maximal aerobic power, PO power output, TT cycling time trial, VO2max maximal oxygen consumption