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Thermolysin reconstruction and segmentation. Top row to bottom: the result of iterative reconstruction using the ToMoBAR package, manual segmentation using Avizo (see Section 3.4), a combined segmentation using GMM (liquor, loop and vacuum) and GeoDistance thresholding for crystal, a combined segmentation using GMM and RegionGrow method for crystal. Image discrepancies between the manual segmentation of the crystal and automated methods are presented in the red boxes.
