Figure 3.

Altered nuclear sirtuin HDAC activity is not detected during Nmnat1-associated disease progression. Quantitative western blot analysis of whole retinal lysate from Nmnat1^V9M/V9M mice and wildtype mice at 3 weeks (n = ;3 per genotype) and 6 weeks (n = ;4 per genotype) was performed using antibodies against histone residues that are targets of nuclear sirtuins. Immunoreactivity against (A) acetylated H3K9 (17 kDa, green), (B) acetylated H3K18 (17 kDa, green) and (C) acetylated H4K16 (11 kDa, green) did not significantly differ by genotype at either age, as indicated by the blots (top) or quantitation of those blots (bottom). (D) The expression level of histone 3 (17 kDa, green) did not differ by genotype at either age, whereas (E) histone 4 expression was significantly lower in Nmnat1^V9M/V9M mice than in wildtype mice at 3 weeks of age (blot, top; quantitation, bottom). The intensities of the target bands were measured relative to β-actin (45 kDa, red). Statistical significance is represented by ^*P < 0.05.