Fig. 3.

Interactome analysis of S1-ΔNTD. (A) Fc-strep or S1-ΔNTD-Fc-strep protein (20 μg/mL) was bound to MagStrep XT beads at 4 °C. Unbound proteins were washed with PBST (0.02% Tween-20). Then, precleared A549 cell lysates were incubated with beads at 4 °C for 4 h. After another washing, the beads were digested with trypsin and subjected to MS. Fc-strep bound proteins were used as controls. (B) S1-ΔNTD-interacting proteins were compared with Fc-interacting proteins in volcano plot using the ggplot2 package in R. Dots with a p value < 0.05 were labeled. ΔNTD specifically bound proteins (fold change > 2) are labeled in red. (C-E) S1-ΔNTD specifically bound proteins were subjected to Gene Ontology analysis using DAVID Bioinformatics Resources 6.8. For each analysis, only top 10 GO terms are displayed. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)