Table 1.

Primer sequences for identifying the expression of target (STAT4, STAT6, GATA3, and T-bet) and reference (ACTB) genes in real-time PCR.

Gene	Forward primer	Reverse primer	Product length (bp)
STAT4	5′-CCCAGAGACTCGAACACTGA-3′	5′-CCTTACAAGAGGCAGCCAGA-3′	203
STAT6	5′-GGCAGGTTCTCTATCGCTCA-3′	5′-GGTCCTCCTCACTCCTCAGA-3′	151
GATA3	5′-GGCGCCGTCTTGATACTT T-3′	5′-CTGGGTAGCGAAGAGCAGAG-3′	188
T-bet	5′-ATGATTGTGCTCCAGTCCC-3′	5′-CCTCTGGCTCTCCGTCGTTC-3′	76
ACTB	5′-AACTTGTGTTGCATGCCTGA-3′	5′-ACCGTTGCCAATCTAAGTGC-3′	176

Abbreviations: STAT4, signal transducer and activator of transcription 4; STAT6, signal transducer and activator of transcription 6; GATA3, GATA binding protein 3; T-bet, T-box expressed in T cell; ACTB, actin beta.