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. 2021 Apr 24;62(3):401–413. doi: 10.1093/jrr/rrab018

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© The Author(s) 2021. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Characterization of EVs released by CAFs. (a) Representative (low magnification) EM micrograph of isolated CAF-EVs, (b) Immunogold and immunofluorescence microscopy analysis of EVs for specific exosomal markers CD9, CD63 and CD81, (c) Size distribution of the generated EVs particles, as determined by a Nano Particle Tracking assay, and (d) Zeta Potential Distribution Analyzer of released CAF-EVs. Scale bars = 200 nm (TEM); 100 nm (immunogold), and 2 μm (SIM).