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. 2021 May 3;11:581172. doi: 10.3389/fonc.2021.581172

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Copyright © 2021 Rasti, Madjd, Saeednejad Zanjani, Babashah, Abolhasani, Asgari and Mehrazma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression Levels of Stemness, EMT, and Angiogenesis-Related Genes and TGF-β1 using qRT-PCR in Parental Cells (PCs) and Spheroid-Derived Cells (SDCs) From Before and After Transfection of PCs with SiSMAD4. (A) Stem cell marker, (B) Mesenchymal marker, (C) ABC transporters, Angiogenesis-related and TGF-β1 genes expression of PCs and SDCs in SFDM and SDCs established by SMAD4 inhibition were quantified by qRT-PCR. Data are represented as mean ± SD (n = 3 each). *P < 0.05, and **P < 0.01.