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Spheroid Derived Cells (SDCs) With Stem Cell-Related Features Before and After SMAD4 Transfection. Sphere formation assay in serum-free defined medium (SFDM) compared to the self-renewal capacity 3D sphere derivatives of the PCs after SiSMAD4 inhibition during 6 days (A:1st to G:7th day). (H) SDCs before transfection with SiSMAD4 showed significantly higher sphere-forming ability after 10 days. Figures are shown with a magnification of 100×.
