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. 2021 May 3;11:581172. doi: 10.3389/fonc.2021.581172

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Copyright © 2021 Rasti, Madjd, Saeednejad Zanjani, Babashah, Abolhasani, Asgari and Mehrazma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression Levels of Stemness, EMT, and Angiogenesis-Related Genes, TGF-β1 and miR204 using qRT-PCR in Spheroid-Derived Cells (SDCs) From Before and After Transfection of PCs with SiSMAD4, cytoplasmic Twist1 expression and survival rates in patients with clear cell Renal Cell Carcinoma. (A) Stem cell markers, (B2) Mesenchymal markers, (C) TGF-β1 gene and (D) (miR204) expression of SDCs in SFDM and SDCs before and after Transfection by SMAD4 inhibition were quantified by qRT-PCR. Data are represented as mean ± SD (n = 3 each). *P < 0.05, **P < 0.01 and ***P < 0.0001. (B1) Correlation between cytoplasmic Twist1 expression and survival rates in patients with clear cell Renal Cell Carcinoma (ccRCC). Progression-free survival (PFS) with cytoplasmic Twist1 expression grouped into low- versus moderate- versus high expression levels.