Table 1. Sequences of primer pairs designed for this work.
| Gene | Locus tag | Forward primer | Reverse primer |
|---|---|---|---|
| aleO | Rmet_5746 | GGAAGACGTCTATCGGACCA | CTGTGGCGTAACAGGATGTC |
| iucA | Rmet_1115 | GCATGTCGTGGATTGATCTG | GTGCAAGGGATATGGCTCAG |
| aleB | Rmet_1118 | ACGAAGAAGGACACGGTCAC | GGGAGATGGCTTGTGTTGTT |
| piuA | Rmet_4617 | TCTCGACGATTTCACGAATG | GCGGTCTCGTCATTGATCTT |
| phaC1 | Rmet_1356 | ACCAATACGACGGCAGAGAG | ACTGAAACGGTTGGGACTTG |
| catA | Rmet_4881 | CGCTGGTAAAGCAGTTCCTC | TTGAACAGATCGGTCGTCAG |
| pcaG | Rmet_4014 | GTCGTTCCGCTTCGTTACC | GCATGAACACCGACACCAT |
| bvgA | Rmet_5714 | CCATTGGAGACTCGCTCTTC | GTCACGACCTTGACCTGCTT |
| phcA | Rmet_2977 | GATGCTGGGCCTGAATATGT | AGAAACTCGCCAACCACCT |