Skip to main content
. 2021 May 17;3(2):zcab018. doi: 10.1093/narcan/zcab018

Figure 4.

Figure 4.

ATR and PARP inhibition cause IDH1/2 mutants to prematurely enter mitosis. (A and B) HCT116 and U87 (WT and IDH1-R132H/+) cells were treated with olaparib (1μM), AZD6738 (500 nM) or both for 24 h. Cell were fixed and stained for p-H3 (Ser10) and counterstained with DAPI. Number of p-H3 positive cells were analyzed in 5 distinct fields (n = 2). (C) Representative p-H3-Alexa-647 plots of HCT116 WT and R132H/+ cells that were treated with olaparib (1 μM), AZD6738 (500 nM) or both for 24 h. DNA content (propidium iodide) and pH3-Ser10/Alexa-488 were assessed by flow cytometry (n = 3). Quantification of result shown on the right panel. (D) Representative EdU-Alexa-647 plots of HCT116 WT and R132H/+ cells were treated with olaparib (1 μM), AZD6738 (500 nM) or both for 24 h DNA content (propidium iodide) and EdU -Ser10/Alexa-488 were assessed by flow cytometry (n = 3). (E) HCT116 (WT and IDH1-R132H/+) cells were treated with olaparib (1 μM), AZD6738 (500 nM) or both for 24 h. Cell were fixed and stained for cyclin-A and counterstained with DAPI. Number of cylin-A positive cells were analyzed in five distinct fields (n = 3). Quantification of result shown on the right panel. Error bars represent means ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05.