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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Nanoscale Horiz. 2021 Apr 22;6(5):393–400. doi: 10.1039/d0nh00679c

Figure 3. Latrunculin A loaded MC ((+)MiNP) co-injection and −4 h pre-injection lead to similar effector particle biodistributions.

Figure 3.

a) Timeline showing the injection times for the co-injection and −4 h injection methods, which were evaluated for both subcutaneous (SC) and intravenous (IV) administration. “(+/−)MiNP” indicated an injection of either (+)MiNP or (−)MiNP, and effector micelle injections are indicated by “E-MC”. All mice were sacrificed at 24 h post E-MC injection. Comparisons of cell uptake in spleen and liver for the different SC (b, c) and IV (d, e) injection methods are shown. In all cases, mice were injected with 100 μL 7μM LatA (+)MiNP or (−)MiNP and E-MC were labelled with DiR for flow cytometric quantification of cellular uptake within the spleen and liver. Data are reported as fold increase median fluorescence intensity of the E-MC over an untreated control. N=5 p<0.0001. To assess the transience of the MiNP effect, mice were injected SC (f) or IV (g) with (+/−)MiNP and E-MC according to the co-injection method, and serum levels of E-MC were evaluated by fluorescence spectroscopy. Mice were then rested for 72 hours and injected again with only E-MC to determine whether the inhibitory effect remained. N=3 for 2 h and 4 h timepoints and N=6 for 24 h timepoints, *p<0.05.