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. 2020 Nov 22;9(2):154–165. doi: 10.1093/gastro/goaa085

Figure 5.

Figure 5.

Sodium butyrate increases the binding capacity of GPR43 and β-arrestin-2, β-arrestin-2 and IкBα. (A) Co-IP was used to detect changes associated with pretreatment with sodium butyrate for 30 min of GPR43 and β-arrestin-2 in RAW264.7cells. (B) Co-IP was used to detect changes associated with pretreatment with sodium butyrate for 30 min of β-arrestin-2 and IкBα in RAW264.7cells, followed by lipopolysaccharide treatment for 6 h. Relative quantitative evaluation of the Western-blot analysis for GPR43/β-arrestin-2, β-arrestin-2/IкBα expression was obtained using Image-Pro Plus 6.0 software.*P < 0.05 vs vehicle group; #P < 0.05 vs LPS group; values are expressed as mean ± SD (n = 6 per group, Bonferroni’s comparison post-hoc test). Co-IP, co-immunoprecipitation.