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. Author manuscript; available in PMC: 2021 May 17.
Published in final edited form as: J Cell Physiol. 2019 Dec 23;235(6):5293–5304. doi: 10.1002/jcp.29415

FIGURE 2.

FIGURE 2

Knockdown of Brd2 and/or Brd4 impairs osteogenic differentiation of MC3T3 cells. (a) Western blot analysis of protein lysates collected 2 days after small interfering RNA (siRNA) transfection. (b) Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis performed on messenger RNA (mRNA) isolated 4 days post-transfection (n = 3, mean ± STD). (c) Pico Sirius Red staining and quantification for collagen deposition performed 7 days after induction of osteogenic differentiation (n = 3, mean ± STD). (d) Alkaline phosphatase (Alpl) enzymatic activity measured on Day 14 of osteogenic differentiation (n = 3, mean ± STD). (e) RT-qPCR conducted on mRNA isolated 3 days after induction of osteogenic differentiation (n = 3, mean ± STD). All error bars represent ± STD of three biological replicates. Statistical significance to nontargeting control group indicated as follows: ^p < .05, *p < .01, #p < .001