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. Author manuscript; available in PMC: 2021 May 17.
Published in final edited form as: J Cell Physiol. 2019 Dec 23;235(6):5293–5304. doi: 10.1002/jcp.29415

FIGURE 5.

FIGURE 5

Continuous +JQ1 treatment prevents osteogenesis of MC3T3 cells. (a) Experimental outline indicating short-term (3 days) and continuous 100 nm +JQ1 treatment in differentiating MC3T3 cells. (b) Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis performed on messenger RNA (mRNA) collected throughout the osteogenic differentiation time course (n = 3, mean ± STD). (c) Representative image (left) and ImageJ quantification (right) of alizarin red staining for hydroxyapatite deposition on Day 24 of osteogenic differentiation. All error bars represent ± STD of three biological replicates. Statistical significance to vehicle (dimethyl sulfoxide [DMSO]) control group indicated as follows: *p < .01, #p < .001