Table 1.
RT-PCR primer sequences used in this study (GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; Ta: annealing temperature)
| Gene name | Regular name | NCBI ID | Primer pairs | Product length | Ta | |
|---|---|---|---|---|---|---|
| Gapdh | Rat GAPDH | NM_017008.4 | Forward: | 5′-CAC CAC CAT GGA GAA GGC TG-3' | 240 bp | 60 °C |
| Reverse: | 5′-GTG ATG GCA TGG ACT GTG-3' | |||||
| Acta2 | Rat alpha smooth muscle actin | NM_031004.2 | Forward: | 5′-GAG CGT GGC TAT TCC TTC GTG-3' | 106 bp | 60 °C |
| Reverse: | 5′-CAG TGG CCA TCT CAT TTT CAA AGT-3' | |||||
| Rn18s | Rat 18S ribosomal RNA | NR_046237.1 | Forward: | 5′-GCG GTC GGC GTC CCC CAA CTT CTT-3' | 105 bp | 60 °C |
| Reverse: | 5′-GCG CGT GCA GCC CCG GAC ATC TA-3' | |||||