Table 2.
List of antibodies used in this study for Western blot (αSMA: alpha smooth muscle actin; pAkt: phospho-protein-kinase B; peNOS: phosphorylated endothelial nitric-oxide synthase, CTGF: connective tissue growth factor)
| Antibody | Vendor | Catalogue number | Dilution | Secondary antibody | Secondary antibody dilution | Secondary incubation time |
|---|---|---|---|---|---|---|
| Anti-αSMA | Sigma–Aldrich, Darmstadt, Germany | A2547 | 1:500 | Goat anti-mouse IgG | 1:6000 | 30 min |
| pAkt | Cell Signaling Technology, Leiden, Netherlands | 4060S | 1:1000 | Goat anti-rabbit IgG | 1:4000 | 30 min |
| Akt | Cell Signaling Technology, Leiden, Netherlands | 9272S | 1:1000 | Goat anti-rabbit IgG | 1:3000 | 1 h |
| peNOS | Cell Signaling Technology, Leiden, Netherlands | 9571S | 1:1000 | Goat anti-rabbit IgG | 1:3000 | 1 h |
| eNOS | Abcam, Cambridge, UK | 66,127 | 1:1000 | Goat anti-rabbit IgG | 1:5000 | 1 h |
| CTGF | Santa Cruz Biotechnology, Dallas, USA | sc-14939 | 1:1000 | Rabbit anti-goat IgG | 1:5000 | 1 h |