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. 2021 May 3;9:625719. doi: 10.3389/fcell.2021.625719

FIGURE 4.

FIGURE 4

Arpin is downregulated in inflamed areas of colon tissue from ulcerative colitis (UC) patients. (A) Western blot for arpin and ArpC5 from resection specimens of patients with UC and non-inflamed controls. β-actin was probed as the loading control. (B) Quantification of pixel intensities including all bands (compare Table 1 for single values; nCtrl = 8, nUC = 10; two-tailed t-test followed by Mann–Whitney test; ns, non-significant. We only excluded sample UC 5 as a technical outlier because of the absence of both arpin and ArpC5 and a much weaker β-actin band. (C) Comparison of pixel intensities from bands of control samples, samples from patients receiving no treatment, and patients receiving mesalazine in their medication regimen. **p < 0.01. (D) Representative immunostaining for occludin and arpin from resection specimens of patients with histologically active UC and non-inflamed controls; n = 3. Bar = 20 μm. (E) Arpin mRNA analysis in biopsies from UC patients compared to controls (n: Control = 11, UC = 10; one-way ANOVA with Kruskal–Wallis correction. (F) mRNA analysis of arpin from publicly available datasets (n: non-inflamed mucosa = 5, inflamed mucosa = 8; two-tailed t-test with Welch’s correction). ***p < 0.001. ns = not significant.