Skip to main content
. 2021 May 3;9:625719. doi: 10.3389/fcell.2021.625719

FIGURE 6.

FIGURE 6

CK666 ameliorates tissue damage in a dextran sulphate sodium (DSS)-induced colitis mouse model. (A) Disease activity index of mice with DSS-induced colitis and treated with either the vehicle dimethylsulfoxide (DMSO) or 5 mg/kg CK666 intraperitoneally (i.p.) starting on day 3 (n = 8–9 per group; two-way ANOVA with Bonferroni’s correction). (B) Colon lengths after DSS-induced colitis (n = 6 per group; one-way ANOVA with Bonferroni’s correction). (C) Hematoxylin/eosin-stained colon tissue sections in 20 × magnification (left) and 40 × (right). Bars = 50 μm (left), 25 μm (right). Crypt dysplasia and ulceration (*), immune cell infiltration (arrow), and edema formation (e) are highlighted; n = 3 per group. (D) Histological inflammation score of colitic mice (n = 3 per group; two-tailed test). (E) Colon permeability to Evans blue in control and colitic mice treated with either DMSO or CK666 (n = 4 per group; one way-ANOVA with Bonferroni’s correction). (F) Immunostaining of zonula occludens 1 (ZO-1) (red) and actin (green) in colon tissue from control and colitic mice treated with DMSO or CK666; n = 3. Bar = 35 μm. *p < 0.05; **p < 0.01; and ***p < 0.001. ns = not significant.