Figure 2.
ER stress can be suppressed by B. dentium, γ-glutamylcysteine, and IL-10. a. qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor thapsigargin. Cells were treated with either media, 50% un-inoculated LDM4 (LDM4), 50% B. dentium LDM4 (Bd), 2 mM γ-glutamylcysteine (yGC), or 100 ng/mL IL-10 (IL-10) (n = 6/experiment). b. qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor tunicamycin (n = 6/experiment). c. Propidium iodide staining of T84 cells after 48 hr incubation with ER stressors (thapsigargin or tunicamycin) or oxidative stressor hydrogen peroxide (H202) (n = 6/experiment). *p < .05, Multi-Way ANOVA