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. 2021 May 17;16(5):e0251354. doi: 10.1371/journal.pone.0251354

Fig 1. ADAADi treatment results in rewiring of ATP-dependent chromatin remodeling protein network.

Fig 1

(A). The transcript levels of BRG1, SMARCAL1, INO80, HELLS, ZRANB3, PICH, and SWR1 was measured using qPCR in HeLa cells after treatment with ADAADi. (B). The transcript levels of CHD1L, CHD1, CHD7, CHD3, BRM, ATRX, BTAF1, EP400, and RAD54L was measured using qPCR in HeLa cells after treatment with ADAADi. (C). The transcript levels of SMARCAL1 and BRG1 in HeLa cells after ADAADi treatment were measured using qPCR. (D). Expression of BRG1 and SMARCAL1 in HeLa cells was monitored by western blot after 24 and 48 hr of treatment. (E). Quantitation of the western blot was done using Image J software and normalized with respect to β-actin. (F). The ATPase activity of SMARCAL1 was measured in untreated and ADAADi-treated HeLa cells. (E). The ATPase activity of BRG1 was measured in untreated and ADAADi-treated HeLa cells. In both these experiments, the percent ATPase activity was measure with respect to the untreated control at the respective time points.