FIGURE 3.

Detection of procoagulant platelets using flow cytometry. A, Platelets are first identified using polygonal gating on the SSC‐A versus CD41a plot and as presenting low SSC and CD41a positivity, and subsequent analyses are based on this gate. B, Gating is further refined in the lower right quadrant by selecting CD41a⁺/CD45^– cells using curved quadrants, and subsequent analyses are based on this gate. The “tail” population (CD41a⁺/CD45⁺) is excluded, as it represents aggregates of platelets and leukocytes. C, Applying straight quadrants to the same platelet population and thresholds for both axes shown in (B) yields slightly different results. D, Thresholds for CD62P and GSAO are set by PE and GSCA isotype controls. E, Procoagulant platelets are identified in the upper‐right quadrants as CD62P⁺/GSAO⁺ cells in a stimulated sample. F, Applying straight quadrants to the same platelet population and thresholds for both axes shown in (E) yields slightly different procoagulant platelet percentages. Images were taken from Tan et al ²⁵