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. 2021 Mar 11;35(5):e23750. doi: 10.1002/jcla.23750

TABLE 2.

Methods used to generate procoagulant platelets described in the literature

Sample types Agonists Conditions Procoagulant platelet ratio
Washed platelets (5 × 104/μL)7 Thrombin (0.1 U/ml) 37°C, 10 min 59.2%
Collagen (5 µg/ml) 37°C, 10 min 45.76%
Thrombin (0.1 U/ml) + collagen (5 μg/ml) 37°C, 10 min 20.78%
Whole blood 25 Thrombin + collagen (final concentrations are variable) Room temperature [25−27°C], 10 min 29.7%
Whole blood 23 Thrombin (2 U/ml) Room temperature [25−27°C], 10 min 13.15%
Collagen (10 μg/ml) Room temperature [25−27°C], 10 min 1.2%
Thrombin (2 U/ml) + collagen (10 μg/ml) Room temperature [25−27°C], 10 min 17.3%
Whole blood (5 × 104/µl) 29 Thrombin (2 U/ml) + collagen (10 μg/ml) 37°C, 10 min 60%