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. 2021 Apr 1;218(1):iyab037. doi: 10.1093/genetics/iyab037

Figure 1.

Figure 1

tof2::Hermes or tof2::kanMX destabilize [URE3]. (A) The original BY241 [URE3-1] strain with a Hermes insertion (shown here to be tof2::Hermes) destabilizing [URE3-1]. A white [URE3-1] single colony of the strain in which [URE3-1] was unstable was streaked for single colonies on YES+W plates. White or pink colonies or sectors are [URE3] and red colonies are [ure-o]. (B) The original Hermes insertion or a deletion/insertion with kanMX (G418-resistance) in each of three strain backgrounds show the same effect as the original Hermes mutant. BY241 is the background that was utilized for the Hermes screen. Σ1278b is often used in studies of nitrogen regulation. Strain 779-6A has been used in studies of [PSI+] and [URE3].