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Elevation of Btn2p and Cur1p in proteasome mutants. Extracts of [ure-o] strains of BY241 with the indicated genotypes were analyzed by western blot. Fold-increase of Btn2p was measured relative to the wild-type with correction for loading using the Pgk1p control. Accurate measurements of Cur1p in the wild-type were impaired by the presence of nearby cross-reacting bands, but the elevation of Cur1p in the proteasomal mutants appears to be of the same order of magnitude as for Btn2p.
