Table 1.

Hermes insertions recovered in proteasome-related genes

Gene	Gene size (nt)	[URE3] –Ade		[ure-o] +Ade
		Insertions	Unique	Insertions	Unique
TOF2	2316	*4120	**21	3723	67
RPN4	1596	**458	**8	3734	67
IRC25	540	**50	*1	269	16
PBA1	917	**1006	**8	6913	46
POC4	447	**0	*0	134	17
HMS3	1443	**179	**5	4019	52
RPN10	807	**0	*0	2665	24
ADD66	804	**349	*5	1574	27
RPN14	1254	10226	*55	7513	106
NAS6	687	**2472	14	3499	29
NAS2	664	2538	10	1940	24
PRE9	777	** 60	1	558	11

Note: The Hermes transposon was mobilized in isogenic [ure-o] and [URE3] cells. After growth, DNA was isolated and insertion sites amplified by PCR, cloned, and sequenced (>57 × 10⁶ reads for [URE3] and >45 × 10⁶ for [ure-o] cultures; Edskes et al. 2018). “Insertions” means total reads within the indicated ORF, while “Unique” indicates distinct sites represented among the insertions in that ORF. Decreased recovery of insertions in a gene in the [URE3] culture could be due to failure of the insertion mutants to stably propagate [URE3] (as is the case for PRE9, for example) or their failure to prevent toxicity of the prion [as was shown for LUG1 (Edskes et al. 2018)]. Data from Edskes et al. (2018). Insertion recovery frequency was less in [URE3] cultures with *P < 0.005, **P  < 0.0001.