Table 3.
Protein level changes in pre9Δ cells (SILAC)
| pre9Δ/WT | StdDev | Gene | Function |
|---|---|---|---|
| 0.14 | 0.06 | PTR2 | Transporter of di- and tri-peptides |
| 0.33 | 0.11 | SST2 | GTPase-activating protein for Gpa1p; regulates desensitization to alpha-factor pheromone |
| 0.36 | 0.15 | STE6 | Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor |
| 0.36 | 0.15 | STE2 | Receptor for alpha-factor pheromone |
| 0.36 | 0.17 | INO1 | Inositol-3-phosphate synthase |
| 0.37 | 0.07 | CWP1 | Cell wall mannoprotein that localizes to birth scars of daughter cells |
| 2.53 | 0.45 | OTU1 | Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p |
| 2.56 | 0.39 | ECM29 | Scaffold protein that assists in association of the proteasome core particle with the regulatory particle |
| 2.57 | 0.69 | APJ1 | HSP40 chaperone with a role in SUMO-mediated protein degradation |
| 2.62 | 1.01 | RTS3 | Putative component of the protein phosphatase type 2A complex |
| 2.71 | 0.55 | HBN1 | Protein of unknown function; similar to bacterial nitroreductases |
| 2.74 | 1.00 | YOR052C | AN1-type zinc finger protein |
| 2.82 | 0.42 | EDC1 | RNA-binding protein that activates mRNA decapping directly |
| 2.94 | 1.67 | SSA3 | HSP70 chaperone |
| 2.94 | 0.50 | CUZ1 | Interacts with ubiquitinated proteins, Cdc48p, and the proteasomal regulatory particle |
| 3.33 | 1.21 | YKR011C | Protein of unknown function |
| 3.37 | 0.73 | HLR1 | Protein involved in regulation of cell wall composition and integrity |
| 3.42 | 1.13 | BIO2 | Biotin synthase; catalyzes the conversion of dethiobiotin to biotin |
| 3.66 | 0.66 | YPL113C | Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates |
| 4.11 | 3.35 | ARO10 | Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde |
| 4.39 | 4.60 | BNA2 | Tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase; required for de novo biosynthesis of NAD from tryptophan |
| 4.52 | 3.24 | PDC5 | Minor isoform of pyruvate decarboxylase |
| 4.94 | 1.85 | TMA10 | Protein of unknown function |
| 4.95 | 0.67 | MAG1 | 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents |
| 4.97 | 3.56 | SNZ1 | Pyridoxal-5-phosphate synthase subunit |
| 5.02 | 1.07 | LPL1 | Phospholipase that maintains lipid droplet (LD) morphology |
| 6.44 | 1.49 | UMP1 | Chaperone required for correct maturation of the 20S proteasome |
| 21.43 | 6.42 | BTN2 | Aggregase and v-SNARE binding protein |
Note: Proteins changed 2.5-fold or more in the pre9Δ strain compared to the WT strain.