Figure 1.

A genome-wide screen identified suppressors of the psh1Δ GALCSE4 SDL. (A) Schematic for the genome-wide screen. A psh1Δ strain (YMB8995) transformed with GAL1-6His-3HA-CSE4 (pMB1458) was mated to an array of nonessential gene deletions and an array of conditional alleles of essential genes. Growth of the haploid meiotic progeny plated in quadruplicate was visually scored on glucose- and galactose-containing media grown at 30°C for nonessential and 26°C for essential gene mutant strains. Ninety-two genes were identified as growing better on galactose-containing media than the psh1Δ GALCSE4 strain. Thirty-eight candidate genes were selected for confirmation of suppression of lethality. (B) Representative plates from the genome-wide screen. Shown is Plate 01 of the nonessential gene deletion array. The mutant strains were spotted in quadruplicate on selective media plates containing glucose (top) or galactose (bottom). The red box highlights psh1Δ hhf1Δ which displayed improved growth on galactose-containing plates compared to the psh1Δ GALCSE4 control strain (perimeter of plate) and did not show a growth defect or improved growth on the glucose plates.