Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 5;218(1):iyab033. doi: 10.1093/genetics/iyab033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by Oxford University Press on behalf of Genetics Society of America 2021.

This work is written by US Government employees and is in the public domain in the US.

PMC Copyright notice

Figure 3. — Deletion of the HHF1 allele reduces enrichment of Cse4 at peri-centromeric and noncentromeric regions. (A–C) ChIP-qPCR was performed on chromatin lysate from wild-type (YMB9804), psh1Δ (YMB10479), hhf1Δ (YMB10937), and psh1Δ hhf1Δ (YMB10822) strains transiently overexpressing GAL1-6His-3HA-CSE4 (pMB1458). Enrichment of 6His-3HA-Cse4 is shown as a fold over wild-type. Displayed are the mean of two independent experiments. Error bars represent standard deviation of the mean. **P < 0.0099, *P < 0.09, ns, not significant. (A and B) Levels of Cse4 enrichment are reduced at noncentromeric regions when HHF1 is deleted. qPCR at A: ACT1, SAP4, RDS1, SLP1, and PHO5 and B: FIG4, COQ3, GUP2, and UGA3. (C) Cse4 enrichment is reduced at peri-centromeric, but not the core centromere, regions in hhf1Δ strains. Top: A diagram of the peri-centromere and centromere of Chromosome III analyzed by ChIP-qPCR. Horizontal lines represent the regions amplified. Bottom: Enrichment of 6His-3HA-Cse4 at the core centromere and the left and right peri-centromeric regions on Chromosome III.