Table 1.
Candidate double mutant strains with the indicated mutant allele combined with psh1Δ GALCSE4 were generated and used for secondary validation using growth assays
| Allele | Systematic name | Gene name | Standard name | Growth score | Colony score | SDL suppression |
|---|---|---|---|---|---|---|
| Nonessential | ||||||
| hhf1Δ | YBR009C | HHF1 | Histone H4 | 3 | 4 | Y |
| hhf2Δ | YNL030W | HHF2 | Histone H4 | 3 | 4 | Y |
| ies2Δ | YNL215W | IES2 | Ino Eighty Subunit | 2 | 3 | N |
| arp8Δ | YOR141C | ARP8 | Actin-Related Protein | 3 | 4 | Y |
| swc5Δ | YBR231C | SWC5 | SWr Complex | 1 | 4 | N |
| eaf1Δ | YDR359C | EAF1 | Esa1p-Associated Factor | 2 | 3 | Partial |
| eap1Δ | YKL204W | EAP1 | EIF4E-Associated Protein | 2 | 4 | Y |
| cse2Δ | YNR010W | CSE2 | Chromosome SEgregation | 2 | 3 | Partial |
| cse2Δ_tsa | YNR010W | CSE2 | Chromosome SEgregation | 3 | 3 | Y |
| mrm2Δ | YGL136C | MRM2 | Mitochondrial rRNA Methyl transferase | 2 | 3 | N |
| hap3Δ | YBL021C | HAP3 | Heme Activator Protein | 3 | 4 | Partial |
| hap5Δ | YOR358W | HAP5 | Heme Activator Protein | 3 | 4 | Y |
| rpl6bΔ | YLR448W | RPL6B | Ribosomal Protein of the Large subunit | 2 | 3 | Y |
| rad4Δ | YER162C | RAD4 | RADiation sensitive | 2 | 3 | Partial |
| rad14Δ | YMR201C | RAD14 | RADiation sensitive | 2 | 2 | Y |
| Essential | ||||||
| act1-132 | YFL039C | ACT1 | ACTin | 3 | 4 | N |
| mob1-5001 | YIL106W | MOB1 | Mps One Binder | 4 | 4 | Y |
| tbf1-5001 | YPL128C | TBF1 | TTAGGG repeat-Binding Factor | 3 | 4 | Y |
| csl4-5001 | YNL232W | CSL4 | Cep1 Synthetic Lethal | 4 | 4 | Y |
| pop4-5001 | YBR257W | POP4 | Processing Of Precursor RNAs | 4 | 4 | Y |
| orc1-5001 | YML065W | ORC1 | Origin Recognition Complex | 4 | 4 | Y |
| orc6-5001 | YHR118C | ORC6 | Origin Recognition Complex | 4 | 4 | Y |
| cft2-1 | YLR115W | CFT2 | Cleavage Factor Two | 3 | 4 | Partial |
| cft2-5001 | YLR115W | CFT2 | Cleavage Factor Two | 4 | 4 | Y |
| clp1-5001 | YOR250C | CLP1 | CLeavage/Polyadenylation factor Ia subunit | 4 | 3 | Y |
| ipa1-5001 | YJR141W | IPA1 | Important for cleavage and PolyAdenylation | 3 | 4 | Y |
| hrp1-1 | YOL123W | HRP1 | Heterogenous nuclear RibonucleoProtein | 2 | 4 | Y |
| rpb5-5001 | YBR154C | RPB5 | RNA Polymerase B | 4 | 4 | Y |
| rpc17-5001 | YJL011C | RPC17 | RNA Polymerase C | 4 | 4 | Y |
| pol31-5001 | YJR006W | POL31 | POLymerase | 4 | 4 | Y |
| srp54-5001 | YPR088C | SRP54 | Signal Recognition Particle 54-kD subunit | 4 | 4 | Y |
| dbp6-5001 | YNR038W | DBP6 | Dead Box Protein | 4 | 4 | Y |
| dbp9-5001 | YLR276C | DBP9 | Dead Box Protein | 4 | 4 | Y |
| yef3-f650s | YLR249W | YEF3 | Yeast Elongation Factor | 2 | 4 | Y |
| cdc5-1 | YMR001C | CDC5 | Cell Division Cycle | 2 | 4 | Y |
| cdc31-1 | YOR257W | CDC31 | Cell Division Cycle | 2 | 4 | Y |
| hrr25-5001 | YPL204W | HRR25 | HO and Radiation Repair | 3 | 4 | Y |
| ost2-5001 | YOR103C | OST2 | OligoSaccharylTransferase | 4 | 4 | Y |
Indicated is the allele analyzed, systematic name, gene name, standard name, visual scoring from the primary screen for growth score (from 1 to 4) and colony score (from 1 to 4), and suppression of SDL (Y: SDL was suppressed; N: SDL was not suppressed; Partial: SDL was partially suppressed).