Figure 2.

Plasma from patients with advanced GC is more prone to stimulate NET formation. (A) Representative immunofluorescence images showing NETs derived from neutrophils that were stimulated by plasma from healthy controls and GC patients with different TNM stages. NETs were characterized by DNA (Hoechst 33342, blue) and neutrophil elastase (NE, green). Scale bar, 20 µm; magnification, ×400. (B) Percentage of cells showing release of NETosis neutrophils conditioned using plasma from each group. These results were representative of 10 experiments. Data are the median and IQR and error bars represent the 10th and 90th percentiles. ns, no significant, ^#P<0.001. NET, neutrophil extracellular trap; GC, gastric cancer.