Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | DH10B | Thermo Fisher | C404010 | Chemically competent |
| Strain, strain background (Saccharomyces cerevisiae) | W303 MATα | https://www.yeastgenome.org/strain/w303 | Chemically and electrocompetent | |
| Recombinant DNA reagent | pCS1748 | PMID:22810204 | Used to characterize ribozyme activity | |
| Sequence-based reagent | PCR and sequencing primers | This paper | PCR primers | Sequences listed in supplementary information |
| Commercial assay or kit | KAPA HiFi PCR Kit | Roche Sequencing Store | KR0368 | |
| Chemical compound, drug | Folinic acid | Millipore Sigma | F7878 | |
| Chemical compound, drug | Theophylline | Sigma Aldrich | T1633 | |
| Chemical compound, drug | Neomycin | Sigma Aldrich | N1876 | |
| Chemical compound, drug | Chloramphenicol | Sigma Aldrich | C0378 | |
| Chemical compound, drug | Tetracycline | Sigma Aldrich | 87128 | |
| Software, algorithm | Python 3 | Python.org | ||
| Software, algorithm | RNAstructure | Reuter and Mathews, 2010 |