Skip to main content
. 2021 May 4;10:e66519. doi: 10.7554/eLife.66519

Figure 2. Optic atrophy 1 (OPA1) brown adipose tissue (BAT) knockout (KO) mice exhibit improved tolerance to cold despite impaired thermogenic activation of BAT.

Figure 2.

(A) Rectal temperature in 8-week-old wild-type (WT) and KO mice exposed to acute cold stress (4°C) over the period of 4 hr. (B–D) mRNA expression of thermogenic genes in BAT of WT and KO mice housed at 30°C or 4°C for 3 days. (B) Relative Ucp1 mRNA levels. (C) Relative Prdm16 mRNA levels. (D) Relative Ppargc1α mRNA levels. mRNA expression was normalized to Gapdh. (E, F) Indirect calorimetry and core body temperature in WT and KO mice exposed to 4°C for 3 days. (E) Core body temperature. (F) Regression plot comparing oxygen consumption as a function of body mass in mice housed at 4°C. (G) Locomotor activity. (H) Food intake (average for each cycle). Data are expressed as means ± SEM. Significant differences were determined by two-Way ANOVA using a significance level of p<0.05. *Significantly different vs. WT mice or vs. 30°C, #significantly different from light cycle or WT mice at 4°C. VO2 data was analyzed by ANCOVA.

Figure 2—source data 1. Optic atrophy 1 (OPA1) brown adipose tissue (BAT) knockout (KO) mice exhibit improved tolerance to cold despite impaired thermogenic activation of BAT.