Figure 5. Optic atrophy 1 (OPA1) deletion in brown adipose tissue (BAT) prevents diet-induced obesity and insulin resistance.

(A–O) Data from wild-type (WT) and OPA1 BAT knockout (KO) mice fed either a control diet (Cont) or a high-fat diet (HFD) for 12 weeks. (A) Total body mass. (B) Percent ratio of fat mass to body mass. (C) Percent ratio of lean mass to body mass. (D) BAT mass. (E) Gonadal white adipose tissue (gWAT) mass. (F) Inguinal white adipose tissue (iWAT) mass. (G) Regression plot comparing oxygen consumption as a function of body mass. (H) Food intake during a 24 hr period. (I) Locomotor activity. (J) Glucose tolerance test (GTT). (K) Area under the curve for the GTT. (L) Fasting glucose levels. (M) Insulin tolerance test (ITT). (N) Area under the curve for the ITT. (O) Fasting insulin levels. Data are expressed as means ± SEM. Significant differences were determined by two-way ANOVA, using a significance level of p<0.05. *Significantly different vs. WT control, #significantly different vs. WT HFD. VO₂ data was analyzed by ANCOVA.

Figure 5—figure supplement 1. Data collected in optic atrophy 1 (OPA1) brown adipose tissue (BAT) knockout mice (KO) and their wild-type littermate controls (WT) fed either control (10% fat content) or high-fat diet (HFD) (60% fat content) for 12 weeks.

Related to Figure 5. (A) Liver mass. (B) Liver triglycerides levels. (C) Serum triglycerides levels. (D) mRNA expression of Ucp1 in BAT. (E) mRNA expression of Ucp1 in inguinal white adipose tissue (iWAT). (F) Representative immunoblot of tyrosine hydroxylase (TH) levels in iWAT of mice fed a HFD and densitometric quantification normalized by tubulin (images were cropped from the same membrane). Data are expressed as means ± SEM. Significant differences were determined by two-way ANOVA, using a significance level of p<0.05. *Significantly different vs. WT control, #significantly different vs. WT HFD.