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. 2021 May 4;10:e66519. doi: 10.7554/eLife.66519

Figure 6. Brown adipose tissue (BAT)-derived fibroblast growth factor 21 (FGF21) does not mediate resistance to diet-induced obesity in optic atrophy 1 (OPA1) BAT knockout (KO) mice.

(A–K) Data from wild-type (WT) and OPA1/FGF21 DKO mice fed either a control diet (Cont) or a high-fat diet (HFD) for 12 weeks. (A) Total body mass. (B) Percent ratio of fat mass to body mass. (C) Percent ratio of lean mass to body mass. (D) Regression plot comparing oxygen consumption as a function of body mass. (E) Food intake during a 24 hr period. (F) Locomotor activity. (G) Glucose tolerance test. (H) Area under the curve for the glucose tolerance test. (I) Fasting glucose levels. (J) Insulin tolerance test. (K) Area under the curve for the insulin tolerance test. Data are expressed as means ± SEM. Significant differences were determined by two-way ANOVA, using a significance level of p<0.05. *Significantly different vs. WT control, #significantly different vs. WT HFD. VO2 data was analyzed by ANCOVA.

Figure 6—source data 1. Brown adipose tissue (BAT)-derived fibroblast growth factor 21 (FGF21) does not mediate resistance to diet-induced obesity in optic atrophy 1 (OPA1) BAT knockout (KO) mice.

Figure 6.

Figure 6—figure supplement 1. Data collected in optic atrophy 1 (OPA1) brown adipose tissue (BAT) knockout mice (KO), OPA1/fibroblast growth factor 21 (FGF21) DKO mice or their respective wild-type littermate controls (WT) fed either control (10% fat content) or high-fat diet (HFD) (60% fat content) for 12 weeks.

Figure 6—figure supplement 1.

Related to Figure 6. (A) BAT mass normalized to body mass. (B) Gonadal white adipose tissue (gWAT) mass normalized to body mass. (C) Inguinal white adipose tissue (iWAT) mass normalized to body mass. (D) Liver triglyceride levels. (E) mRNA expression of Ucp1 in BAT normalized to Gapdh. (F) mRNA expression of Ucp1 in iWAT. (G) Representative immunoblot of tyrosine hydroxylase (TH) levels in iWAT of mice fed a HFD and densitometric quantification normalized by tubulin (images were cropped from the same membrane). (H) Serum FGF21 level in WT and DKO mice. (I) Serum FGF21 levels in WT and OPA1 BAT KO mice (KO). Data are expressed as means ± SEM. Significant differences were determined by two-way ANOVA, using a significance level of p<0.05. *Significantly different vs. WT control, #significantly different vs. WT HFD.
Figure 6—figure supplement 1—source data 1. Data collected in optic atrophy 1 (OPA1) brown adipose tissue (BAT) knockout mice (KO), OPA1/fibroblast growth factor 21 (FGF21) DKO mice or their respective wild-type littermate controls (WT) fed either control (10% fat content) or high-fat diet (HFD) (60% fat content) for 12 weeks.