Figure 8. Activating transcription factor 4 (ATF4) induction in brown adipose tissue (BAT) is necessary for the resistance to diet-induced obesity (DIO) and insulin resistance (IR) in optic atrophy 1 (OPA1) BAT knockout (KO) mice.

(A–K) Data from wild-type (WT) and OPA1/ATF4 BAT DKO mice fed a high-fat diet (HFD) for 12 weeks. (A) Total body mass. (B) Percent ratio of fat mass to body mass. (C) Percent ratio of lean mass to body mass. (D) Glucose tolerance test. (E) Area under the curve for the glucose tolerance test. (F) Fasting glucose levels. (G) Insulin tolerance test. (H) Area under the curve for the insulin tolerance test. (I) Fasting insulin levels. (J) Liver mass normalized to body mass. (K) Liver triglycerides levels. Data are expressed as means ± SEM. Significant differences were determined by Student's t‐test, using a significance level of p<0.05.