Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (mouse, C57Bl/6J) | Murine models | Jackson Laboratories | JAX Stock #024670 RRID:IMSR_JAX:024670 |
Tg (Ucp1-cre)1Evdr; male and female |
| Strain, strain background (mouse, C57Bl/6J) | Murine models | Jackson Laboratories | JAX Stock #025124 RRID:IMSR_JAX:025124 |
C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J; male and female |
| Antibody | Anti-OPA1 (Mouse monoclonal) | BD Biosciences | #612606 RRID:AB_399888 |
WB (1:1000), primary |
| Antibody | Anti-FGF21(Rabbit monoclonal) | Abcam | #ab171941 | WB (1:1000), primary |
| Antibody | Anti-GAPDH (Rabbit monoclonal) | Cell Signaling Technology | #2118 RRID:AB_561053 |
WB (1:1000), primary |
| Antibody | Anti-VDAC (Rabbit polyclonal) | Thermo Scientific | #PA1‐954A RRID:AB_2304154 |
WB (1:1000), primary |
| Antibody | Anti-UCP1 (Rabbit polyclonal) | Abcam | #Ab10983 RRID:AB_2241462 |
WB (1:1000), primary Histology 1:250 |
| Antibody | Anti-SDH (Mouse monoclonal) | Abcam | #Ab14714 | WB (1:1000), primary |
| Antibody | Anti-α-tubulin (Mouse monoclonal) | Sigma | #T9026 | WB (1:1000), primary |
| Antibody | Anti-β-actin (Rabbit polyclonal) | Sigma | #A2066 RRID:AB_476693 |
WB (1:1000), primary |
| Antibody | Anti-tyrosine hydroxylase (Rabbit polyclonal) | Cell Signaling Technology | #2792 RRID:AB_2303165 |
WB (1:1000), primary |
| Antibody | Anti-phosphorylated eIF2α serine 51 (Rabbit monoclonal) | Cell Signaling Technology | #3597 | WB (1:1000), primary |
| Antibody | anti-eIF2α (Mouse monoclonal) | Santa Cruz Biotechnology | #SC81261 | WB (1:1000), primary |
| Antibody | IRDye 800CW anti‐mouse | LI-COR | #925‐32212 RRID:AB_2716622 |
WB (1:10,000), secondary |
| Antibody | Alexa Fluor anti‐rabbit 680 | Invitrogen | #A27042 | WB (1:10,000), secondary |
| Antibody | Anti-rabbit biotinylated secondary antibody | Cell Signaling Technology | #14708 | Histology (1:500) |
| Chemical compound, drug | 5-hydroxytamoxifen | Sigma | T176 | Used in vitro |
| Commercial assay or kit | RNeasy kit | Qiagen Inc | #74104 | |
| Commercial assay or kit | EnzyChrom Triglyceride Assay Kit | BioAssay Systems | #ETGA-200 | |
| Commercial assay or kit | Mouse/rat fibroblast growth factor 21 ELISA | Biovendor | #RD291108200R | |
| Commercial assay or kit | Ultra-Sensitive Mouse Insulin ELISA Kit | Chrystal Chem | #90080 | |
| Commercial assay or kit | High-Capacity cDNA reverse Transcription Kit | Applied Biosystems | #4368814 | |
| Commercial assay or kit | Hematoxylin and Eosin Stain Kit | Vector Laboratories | #H3502 | |
| Software, algorithm | GraphPad Prism Software | GraphPad Software, La Jolla, CA, USA | Version 8.0.0 for Windows RRID:SCR_002798 |
|
| Other | 2920X, standard chow | Harlan Teklad | 2920X | |
| Other | Chow, 60% HFD | Research Diets | D12492 | |
| Other | Chow, 10% Control | Research Diets | D12450J | |
| Sequence-based reagent | Fgf21_F | Integrated DNA Technologies, Inc | PCR primers | TGACGACCAAGACACTGAAGC |
| Sequence-based reagent | Fgf21_R | Integrated DNA Technologies, Inc | PCR primers | TTTGAGCTCCAGGAGACTTTCTG |
| Sequence-based reagent | Atf4_F | Integrated DNA Technologies, Inc | PCR primers | AGCAAAACAAGACAGCAGCC |
| Sequence-based reagent | Atf4_R | Integrated DNA Technologies, Inc | PCR primers | ACTCTCTTCTTCCCCCTTGC |
| Sequence-based reagent | Chop_F | Integrated DNA Technologies, Inc | PCR primers | GTCCCTAGCTTGGCTGACAGA |
| Sequence-based reagent | Chop _R | Integrated DNA Technologies, Inc | PCR primers | TGGAGAGCGAGGGCTTTG |
| Sequence-based reagent | Ern1_F | Integrated DNA Technologies, Inc | PCR primers | TGAAACACC CCTTCTTCTGG |
| Sequence-based reagent | Ern1_R | Integrated DNA Technologies, Inc | PCR primers | CCT CCT TTT CTA TTC GGT CAC TT |
| Sequence-based reagent | Opa1_F | Integrated DNA Technologies, Inc | PCR primers | ATACTGGGATCTGCTGTTGG |
| Sequence-based reagent | Opa1_R | Integrated DNA Technologies, Inc | PCR primers | AAGTCAGGCACAATCCACTT |
| Sequence-based reagent | Ucp1_F | Integrated DNA Technologies, Inc | PCR primers | GTGAAGGTCAGAATGCAAGC |
| Sequence-based reagent | Ucp1_R | Integrated DNA Technologies, Inc | PCR primers | AGGGCCCCCTTCATGAGGTC |
| Sequence-based reagent | Prdm16_F | Integrated DNA Technologies, Inc | PCR primers | CAGCACGGTGAAGCCATTC |
| Sequence-based reagent | Prdm16_R | Integrated DNA Technologies, Inc | PCR primers | GCGTGCATCCGCTTGTG |
| Sequence-based reagent | Gapdh_F | Integrated DNA Technologies, Inc | PCR primers | AACGACCCCTTCATTGAC |
| Sequence-based reagent | Gapdh_R | Integrated DNA Technologies, Inc | PCR primers | TCCACGACATACTCAGCAC |
| Sequence-based reagent | Ppargc1a_F | Integrated DNA Technologies, Inc | PCR primers | GTAAATCTGCGGGATGATGG |
| Sequence-based reagent | Ppargc1a_R | Integrated DNA Technologies, Inc | PCR primers | AGCAGGGTCAAAATCGTCTG |
| Sequence-based reagent | Dio2_F | Integrated DNA Technologies, Inc | PCR primers | AATTATGCCTCGGAGAAGACCG |
| Sequence-based reagent | Dio2_R | Integrated DNA Technologies, Inc | PCR primers | GGCAGTTGCCTAGTGAAAGGT |
| Sequence-based reagent | Nrg4_F | Integrated DNA Technologies, Inc | PCR primers | ACTCACTAAGCCAGAGTGAAGCAGG |
| Sequence-based reagent | Nrg4_R | Integrated DNA Technologies, Inc | PCR primers | CATGTCGTCTCTACAGGTGCTCTGC |
| Sequence-based reagent | Cre_F | Integrated DNA Technologies, Inc | PCR primers | AATGCTTCTGTCCGTTTGCC |
| Sequence-based reagent | Cre_R | Integrated DNA Technologies, Inc | PCR primers | ACATCTTCAGGTTCTGCGGG |
| Sequence-based reagent | Cpt1b_F | Integrated DNA Technologies, Inc | PCR primers | TGCCTTTACATCGTCTCCAA |
| Sequence-based reagent | Cpt1b_R | Integrated DNA Technologies, Inc | PCR primers | AGACCCCGTAGCCATCATC |
| Sequence-based reagent | Ppara_F | Integrated DNA Technologies, Inc | PCR primers | GAGAATCCACGAAGCCTACC |
| Sequence-based reagent | Ppara_R | Integrated DNA Technologies, Inc | PCR primers | ATTCGGACCTCTGCCTCTTT |
| Sequence-based reagent | Acadm_F | Integrated DNA Technologies, Inc | PCR primers | ACTGACGCCGTTCAGATTTT |
| Sequence-based reagent | Acadm_R | Integrated DNA Technologies, Inc | PCR primers | GCTTAGTTACACGAGGGTGATG |
| Sequence-based reagent | Metrnl_F | Integrated DNA Technologies, Inc | PCR primers | CTGGAGCAGGGAGGCTTATTT |
| Sequence-based reagent | Metrnl_R | Integrated DNA Technologies, Inc | PCR primers | GGACAACAAAGTCACTGGTACAG |
| Sequence-based reagent | Bmp8b_F | Integrated DNA Technologies, Inc | PCR primers | CAACCACGCCACTATGCA |
| Sequence-based reagent | Bmp8b_R | Integrated DNA Technologies, Inc | PCR primers | CACTCAGCTCAGTAGGCACA |
| Sequence-based reagent | Slit2-c_F | Integrated DNA Technologies, Inc | PCR primers | GCTGTGAACCATGCCACAAG |
| Sequence-based reagent | Slilt2-c_R | Integrated DNA Technologies, Inc | PCR primers | CACACATTTGTTTCCGAGGCA |
| Sequence-based reagent | Evlov6_F | Integrated DNA Technologies, Inc | PCR primers | TCAGCAAAGCACCCGAAC |
| Sequence-based reagent | Evlov6_R | Integrated DNA Technologies, Inc | PCR primers | AGCGACCATGTCTTTGTAGGAG |
| Sequence-based reagent | Il6_F | Integrated DNA Technologies, Inc | PCR primers | TGGGAAATCGTGGAAATGAG |
| Sequence-based reagent | Il6_R | Integrated DNA Technologies, Inc | PCR primers | GAAGGACTCTGGCTTTGTCTT |