Figure 2.

Neferine may reduce neuron loss and promote white matter recovery. (a) Representative images of Nissl staining in the cortex and hippocampus CA1, CA3, and DG in the injured ipsilateral brain 7 d post-HI. The columns on the right show magnified images of the black boxes in the left column. Scale bar = 100 μm. (b) Protein expression level of microtubule-associated protein 2 (MAP-2) and myelin basic protein (MBP) 24 h after HI brain injury 7 d post-HI. (c, d) Analyses of MAP-2 and MBP (of β-actin). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. n = 4. (e) Representative images of immunohistochemical staining of MAP-2 and MBP 7 d post-HI. Scale bar = 1 mm.