The NOLC1 expression in ESCA tissues and cell lines. (a) The expression pattern of NOLC1 in ESCA tissues and normal esophageal epithelial tissues was analyzed by GEPIA database. (b) The NOLC1 mRNA in ESCC tissues and adjacent normal tissues was measured by QRT-PCR. (c) The NOLC1 expression in ESCC tissues was evaluated by immunohistochemical staining: (1) No expression, (2) high expression. (d) The NOLC1 expression in five esophageal cancer cell lines (EC9706, Eca109, TE-13, Kyse170, T.TN) and normal esophageal cell line HEEC was detected by QRT-PCR. vs si-NC group, ∗∗p < 0.05, ∗∗∗∗p < 0.01. (e) The NOLC1 expression in five esophageal cancer cell lines (EC9706, Eca109, TE-13, Kyse170, T.TN) and normal esophageal cell line HEEC was detected by western blot.